Composite

Part:BBa_K4192131:Design

Designed by: Jiahui Li   Group: iGEM22_CAU_China   (2022-09-30)


yf1(N37C)-RBS-fixJ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1141
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1141
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 1230
    Illegal NgoMIV site found at 1523
    Illegal NgoMIV site found at 1617
    Illegal AgeI site found at 258
    Illegal AgeI site found at 1398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1287
    Illegal BsaI.rc site found at 157


Design Notes

This is a key part to light regulation system including a mutated yf1 and a normal fixJ.


Source

Used a mutated yf1 to produce better regulation of gene expression.

References